Recombinant vesicular stomatitis virus (rVSV) pseudoparticles displaying foreign glycoproteins are valuable for various applications, including vaccine vectors, oncolytic viruses, and research tools. Replication incompetent rVSV pseudoparticles, which do not encode their envelope proteins necessary for infection, offer increased versatility and rapid manufacturing. Traditionally, pseudotyping has been

More precise base editors make mitochondrial DNA editing efficient enough to model disease and correct pathogenic mutations in rodents, but they are slow to move into clinical trials.

Real‐time multivariate statistical process monitoring (RT‐MSPM) is essential to monitor health of bio‐pharmaceutical processes and detect anomalies and faults early in the process. RT‐MSPM methods are commonly used to monitor cell culture process operations in biologics drug substance manufacturing. Batch evolution models (BEMs) are among common RT‐MSPM methods. As an alternative to BEMs, it is possible

Merck’s new antibody clesrovimab and other long-lasting shots promise to both expand and simplify care of newborns across the world to combat respiratory syncytial virus.

We read with interest the Comment that suggests a taxonomy for brain–computer interfaces (BCIs) based on application1, and we agree that a nomenclature of different types of BCI may be useful for discussing this broad and expanding field comprised of stakeholders with different backgrounds and expertise, including groups new to BCI, such as media, investors and students. Nevertheless, we feel compelled

There is increasing interest in the development of intensified processes that can provide significant reductions in manufacturing costs for monoclonal antibody (mAb) products. This study examines the performance of an integrated membrane process that combines single‐pass tangential flow filtration (SPTFF) with high‐performance countercurrent membrane purification (HPCMP) for the preconditioning of

Technologies for large‐scale manufacturing of viral vectors for gene therapies, such as tangential flow filtration and membrane chromatography, are under development. In these early stages of process development, techno‐economic analyses are useful for identifying membrane properties yielding the greatest impact on process performance. In this study, we adapted a techno‐economic framework used for

Hyaline cartilage is a dense avascular tissue with low regenerative potential, present at the ends of the diarthrodial joints and in the cartilage growth plate. Skeletal diseases often result from extracellular changes in this tissue; however, studies of these are hindered by the tissue complexity, the difficulty in obtaining human material, and the cost of generating animal models. Recent developments

The gut microbiota plays an essential role in host health by regulating gut barrier function and immune system homeostasis. However, research into the physiological and immunological functions of the gut microbiota using In Vitro models that mimic the immune environment of the gut remains limited. Herein, we developed the Gut Microbial Immune & Epithelial Cellular System (GutMICS), a device for coculturing

Komagataella phaffii, also known as Pichia pastoris, is a powerful host for recombinant protein production, in part due to its exceptionally strong and tightly controlled PAOX1 promoter. Most K. phaffii bioprocesses for recombinant protein production rely on PAOX1 to achieve dynamic control in two‐phase processes. Cells are first grown under conditions that repress PAOX1 (growth phase), followed by

In Europe, Advanced Therapy Medicinal Products (ATMPs) include medicines based on gene therapy, somatic-cell therapy, tissue-engineered products, and combined ATMPs. ATMPs constitute an emerging and innovative class of medicines used to treat multiple pathologies and are particularly relevant in pathologies where therapeutic options are limited and require high medical needs. These therapies act, among

Rapid, accurate, cost-effective, and efficient ultrasensitive detection strategies are essential for public health safety (including food safety, disease prevention and environmental governance). The CRISPR/CRISPR-associated (Cas) detection is a cutting-edge technology that has been widely used in the detection of public health safety due to its targeted cleavage properties (signal amplification),

The increasing nitrogen emissions from industrial and agricultural activities have exacerbated pollution in aquatic ecosystems, highlighting a great need for effective nitrogen removal strategies. Genetic engineering has proven to be an effective strategy to improve the nitrogen removal capacity of denitrifying bacteria. However, the widespread use of antibiotics in the genetic modification of denitrifying

In recent years, the environmental unsustainability of traditional wastewater treatment processes, alongside the growing emphasis on carbon neutrality and self-sufficiency in wastewater treatment plants (WWTPs), has drawn attention to algal-bacterial aerobic granular sludge (AGS) systems. This review synthesizes recent advances in algal-bacterial AGS, focusing on the latest research on its granulation

In aquaculture, the presence of antibiotic residues is an escalating concern that poses significant risks to human health. Establishing efficient and user-friendly detection methods for antibiotic residues has become crucial to mitigate these potential hazards. To address the limitations of traditional techniques, novel enzyme biosensors, offering high sensitivity and specificity and rapid response

Isoprenol (3-methyl-3-buten-1-ol) is a precursor to aviation fuels and other commodity chemicals and can be microbially synthesized from renewable carbon streams. Its production has been demonstrated in Pseudomonas putida KT2440 but its titers, rates, and yields have yet to reach commercially viable levels, potentially due to toxicity to the bacterial chassis. We hypothesized that utilization of Tolerization

Site-specific retroelements, such as R2 retrotransposons, have potential as programmable genome-editing systems, but so far have only been used for targeted insertion at genomic safe-harbor loci. In a paper now published in Nature, Fell et al. profile the evolution of site-specific retrotransposon families to gain insight into target site preferences, which they then used to engineer reprogramming

Huanglongbing is a devastating bacterial disease of citrus trees with no known cure. An emerging strategy to provide resistance to plant pathogens is disrupting genes that increase disease susceptibility. A paper in Science by Zhao et al. uses this approach in citrus trees, and identifies a regulatory circuit that can be modulated to alleviate Huanglongbing disease symptoms. Huanglongbing can infect

Recent patents relating to systems, methods and compositions for bioprinting.

Regulatory DNA sequences orchestrate cell-type-specific gene expression by facilitating transcription factor binding, yet their precise effects and reprogrammability remain challenging to delineate. Now, in Cell, Martyn et al. develop a method called variant effects from flow-sorting experiments with CRISPR targeting screens (Variant-EFFECTS), which measures the quantitative effects of changes to regulatory

Polybion creates this next-generation material by feeding a mango fruit waste slurry to Acetobacter bacteria genetically modified to speed up their metabolism. The bacteria feed on the sugar and turn it into cellulose to form a gel-like mat on the surface of the fermentation tank. Bacteria employ this strategy to survive in harsh environments. “It’s a shelter they create [against] the radiation of

Recent moves of note in and around the biotech and pharma industries.

Big pharma and investors are piling into the precision radiation space as the next generation of increasingly potent targeted α-particle therapies promises to destroy cancerous cells with minimum damage to healthy tissues.

In late March, the US Food and Drug Administration’s (FDA) director of the Center for Biologics Evaluation and Research, Peter Marks, resigned under pressure from Health and Human Services Secretary Robert F. Kennedy. In his resignation letter Marks said he had been asked to hand in data on measles vaccine-related deaths and brain swelling that do not exist.

Brazil is the first country in Latin America to produce its own cut-price chimeric antigen receptor (CAR)-T cell therapies. Now, Caring Cross, a US-based non-profit focused on making advanced therapies affordable, plans to expands Brazil’s model to Turkey and the Middle East.

As part of its Dare2eraD TB initiative to combat the challenge of drug-resistant tuberculosis, Indian researchers sequence the genomes of 10,000 Mycobacterium tuberculosis isolates. Sequencing will aid in personalizing antibiotic prescription, allowing faster, more effective treatment. India has set the goal of eliminating TB by 2025, five years ahead of the World Health Organization’s global target