Single-cell electrorotation (ROT) has emerged as a fundamental technique for characterizing cellular electrical properties, yet conventional methodologies face significant limitations including laborious cell loading procedures, time-consuming measurements, low throughput, and confined effective operational regions. To address these challenges, we present an innovative pump-free single-cell electrorotation

Disulfide-rich peptides (DRPs) have evolved intricate topologies to carry out a wide range of bioactivities throughout nature, e.g., in fungi, insects, plants and animals, and have proven applications in medicine and agriculture. To discover novel DRPs, it is now routine to screen DRP libraries for target affinity, but target binding does not necessarily correlate with function. This study reports

In this study, we present a genetic diagnostic device with a four-stepwise logarithmic dilution capability for rapid and reliable detection of target nucleic acids in a single operation using the colorimetric loop-mediated isothermal amplification (LAMP) method. An innovative feature is the confluent point with differing microchannel heights ensuring the synchronized inflow of liquids while preventing

Preclinical ex vivo models capable of probing patient-specific tumor-immune interactions are particularly attractive candidates for interrogating mechanisms of resistance, developing predictors of response as well as assessing next-generation immunotherapeutics. By maintaining features of a patient’s own tumor microenvironment, such patient-derived ex vivo models are poised to meaningfully contribute

Ion concentration polarization focusing (ICPF) is an electrokinetic technique that has shown promise in achieving even billion-fold preconcentration factors. However, increasing the volumetric throughput of ICPF is challenging because disruptive processes that reduce preconcentration efficiency worsen as the channel cross-section extends beyond the microscale. We previously introduced an approach for

The discovery of monoclonal antibodies (mAbs) is critical to advancing therapeutics, diagnostics, and biomedical research. While mouse-derived mAbs dominate current applications, their limitations—short serum half-life, human immunogenicity, and restricted recognition of human-specific antigens—highlight the need for alternative sources. Rabbit-derived mAbs have been gaining significant traction with

Afterglow electrochemiluminescence (ECL), persistent luminescence observed after cessation of electric excitation, has attracted great attention but remains unexplored in sensing application. Here we present a solid-state afterglow ECL biosensor leveraging enzyme-responsive polymer nanoarchitectonics for lipase quantification. The sensor is constructed by covalently grafting amphiphilic block copolymers

Nanoprobe-based biosensors have emerged as a promising alternative to conventional analytical methods, offering advantages in rapid detection, cost-effectiveness, and no need for professional participation. However, challenges in nanoprobe complexity and instability impede biosensor standardization, frequently resulting in unreliable results. To address these limitations, we present a novel signal-compensated

Photoelectrochemical (PEC) sensors have attracted significant attention due to their high sensitivity and rapid response. However, traditional PEC sensors are based on the principle that the target directly or indirectly modulates Faradaic reactions on the photoelectrode surface, making them highly susceptible to interference from complex biological samples. In this study, we employed vacuum filtration

Glutathione (GSH), a pivotal antioxidant, plays a critical role in Parkinson’s disease (PD) pathogenesis, where oxidative stress drives neuronal damage. However, the dynamic changes of GSH in the brain remain poorly understood. Here, we develop a cyclometalated iridium complex (Ir-DO) for GSH monitoring via multiphoton phosphorescence lifetime imaging. Ir-DO exhibits exceptional optical properties

Drug-facilitated crimes (DFC) involve the administration of illicit substances, leaving traces in biological samples within particular period of time. A reliable method for detecting and monitoring illicit drug in biofluids is critical for forensic and clinical applications. Herein, we introduce the hotspot-driven molecular entrapment (HDME) method as highly sensitive strategy for trapping and label-free

Nerve agents pose a significant threat to public health and security, necessitating the development of rapid and highly sensitive detection technologies. Dimethyl methylphosphonate (DMMP), a widely used simulant of sarin, serves as a benchmark for evaluating sensor performance. In this work, we report a single-walled carbon nanotube field-effect transistor (CNTFET) gas sensor fabricated via electrostatic

Mercury is a harmful heavy metal that gravely threatens the environment and organisms. In the current study, probes 2-(1,3-oxathiolan-2-yl)-1,3-diphenylindolizine (DPIC-OS) and 2-(1,3-dithiolan-2-yl)-1,3-diphenylindolizine (DPIC-SS) are designed by protecting a 1,3-diphenylindolizine-2-carbaldehyde (DPIC-CHO) with a mercury-recognizable 1,3-dithiolane and 1,3-oxathiolane moieties and utilized for the

The quantitative distribution of various clusters in molten binary K2O·nTiO2 (n = 0.5, 1, 2, 3, 4, and 6) was investigated by in situ high-temperature Raman spectroscopy and quantum chemical (QC) ab initio calculation. A series of titanium-oxygen model clusters were constructed, and their symmetric stretching vibrational bands and the corresponding Raman scattering cross-sections of titanium nonbridging

Accurate diagnosis and effective treatment of primary liver tumors are crucial, and optical imaging is widely used in clinical image-guided surgery. The poor stability of traditional dyes and the non-specific binding of proteins can easily lead to misdiagnosis in qualitative assays. We have developed a protein-actived nano-fluorescent probe, ND-TPE-COOH, to label normal liver cells/tissues safely for