Improving the methionine (Met) content in maize kernels is of key importance to the animal feed industry; however, the genetic and molecular mechanisms governing maize kernel Met content remain largely unexplored. In this study, we leveraged a panel consisting of 348 diverse inbred maize lines to explore the genetic and molecular mechanisms that control kernel Met levels. A genome-wide association

RNA degradation systems (e.g., RNA decay and RNA interference) and the phytohormone abscisic acid (ABA) are both essential for plant growth, development, and adaptation to stress. Although the interplay between these pathways has been recognized, the molecular mechanisms governing their coordination remain poorly understood. In this study, we revealed that mutations in the 5′–3′ RNA-degrading enzyme

Glyceollins are phytoalexins, soybean-produced compounds that respond to pathogen invasion, injury, and environmental challenges. While these compounds have diverse bioactivities, their limited accessibility hinders further physiological and biochemical studies. Additionally, the incomplete understanding of glyceollin biosynthesis, particularly cyclization steps, remains a major barrier to sustainable

Drought severely threatens food security, and its detrimental effects will be exacerbated by climate change in many parts of the world. Rice production is water-consuming and particularly vulnerable to drought stress. Upland rice is a special rice ecotype that specifically adapts to dryland mainly due to its robust root system. However, the molecular and developmental mechanism underlying this adaption

ABA INSENSITIVE 1 (ABI1) and ABI2 are co-receptors of the phytohormone abscisic acid (ABA). Studies have demonstrated that phosphorylation of multiple amino acids on ABI1/2 augments their ability to inhibit ABA signaling in planta. However, whether and how the dephosphorylation of ABI1/2 is regulated to enhance plant sensitivity to ABA remain unknown. In this study, we identified two protein phosphatases

Coordinated gene transcription in plastids and nuclei is necessary for the photosynthetic apparatus assembly during chloroplast biogenesis. Despite the identification of several transcription factors regulating nuclear-encoded photosynthetic gene transcription, transcription factors regulating plastid gene transcription are barely reported. Here, we report that BAI1 (“albino” in Chinese), a nucleus–plastid

Chemical defoliation stands as the ultimate tool in enabling the mechanical harvest of cotton, offering economic and environmental advantages. However, the underlying molecular mechanism that triggers leaf abscission through defoliant remains unsolved. In this study, we meticulously constructed a transcriptomic atlas through single-nucleus mRNA sequencing (snRNA-seq) of the abscission zone (AZ) from

Genetic transformation is a crucial tool for investigating gene function and advancing molecular breeding in crops, with Agrobacterium tumefaciens-mediated transformation being the primary method for plant genetic modification. However, this approach exhibits significant genotypic dependence in maize. Therefore, to overcome these limitations, we combined dynamic transcriptome analysis and genome-wide

Modern cultivated rice plays a pivotal role in global food security. China accounts for nearly 30% of the world’s rice production and has developed numerous cultivated varieties over the past decades that are well adapted to diverse growing regions. However, the genomic bases underlying the phenotypes of these modern cultivars remain poorly characterized, limiting the exploitation of this vast resource

Sorghum, the fifth most important food crop globally, is a source of silage forage, fiber, syrup, and biofuel. Moreover, it is widely recognized as an ideal model crop for studying stress biology becaused of its ability to tolerate multiple abiotic stresses, including high salt-alkali conditions, drought, and heat. However, functional genomics studies on sorghum have been challenging, primarily due

Plant cells sense H2O2, a major reactive oxygen species (ROS), through cysteine oxidation of critical immune regulators. Recently, Liao et al. reported that the rice transcription factor bHLH25 directly perceives pathogen-induced H2O2 via methionine oxidation to trigger defense responses. This study highlights the importance and complexity of H2O2-mediated oxidation modification in plant immunity.

Genome editing using CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein) or other systems has become a cornerstone of numerous biological and applied research fields. However, detecting the resulting mutations by analyzing sequencing data remains time consuming and inefficient. In response to this issue, we designed SuperDecode, an integrated software toolkit

Human adiponectin receptors (AdipoRs) and membrane progestin receptors (mPRs, members of the progestin and adipoQ receptor [PAQR] family) are seven-transmembrane receptors involved in the regulation of metabolism and cancer development, which share structural similarities with G protein-coupled receptors. Plant PAQR-like sensors (PLSs) are homologous to human PAQRs but their molecular functions remain

Edible maize is an important food crop that provides energy and nutrients to meet human health and nutritional requirements. However, how environmental pressures and human activity have shaped the metabolome of edible maize remains unclear. In this study, we collected 452 diverse edible maize accessions worldwide, including waxy, sweet, and field maize. A total of 3020 non-redundant metabolites, including

Soybean is one of the most important crops globally, and its production must be significantly increased to meet increasing demand. Elucidating the genetic regulatory networks underlying soybean organ development is essential for breeding elite and resilient varieties to ensure increased soybean production under climate change. An integrated transcriptomic atlas that leverages multiple types of transcriptomics

A complete reference genome assembly is crucial for biological research and genetic improvement. Owing to its large size and highly repetitive nature, there are numerous gaps in the globally used wheat Chinese Spring (CS) genome assembly. In this study, we generated a 14.46 Gb near-complete assembly of the CS genome, with a contig N50 of over 266 Mb and an overall base accuracy of 99.9963%. Among the

Plant cell expansion is regulated by hormones and driven by turgor pressure, which stretches the cell wall and can potentially cause wall damage or rupture. How plant cells avoid cell wall rupture during hormone-induced rapid cell expansion remains poorly understood. Here, we show that the wall-sensing receptor kinase FERONIA (FER) plays an essential role in maintaining cell wall integrity during brassinosteroid

Methane in rice paddies is mainly produced by methanogenic communities feeding on carbon from root exudates and debris. However, the dominant root secretion governing methane emissions is not yet identified after decades of studies, even though secreted carbohydrates and organic acids have been shown to contribute to methane emissions. In this study, we discovered that fumarate and ethanol are two

The integration of genotypic and environmental data can enhance genomic prediction accuracy for crop field traits. Existing genomic prediction methods fail to consider environmental factors and the real growth environments of crops, resulting in low genomic prediction accuracy. In this work, we developed GEFormer, a genotype–environment interaction genomic prediction method that integrates gating multilayer

Activities of the chloroplasts and nucleus are coordinated by retrograde signaling, which play crucial roles in plant development and environmental adaptation. However, the connection between chloroplast status and nuclear genome stability is poorly understood. Chloroplast state transitions enable the plant to balance photosystem absorption capacity in an environment with changing light quality. Here

Potato is the world’s most important nongrain crop. In this study, to assess genetic diversity within the Petota section, 29 genomes from Petota and Etuberosum sections were newly de novo assembled and 248 accessions of wild potatoes, landraces, and modern cultivars were re-sequenced at >25× depth. Subsequently, a graph-based pangenome was constructed using DM8.1 as the backbone, integrating194,330

Plants possess remarkably durable resistance against non-adapted pathogens in nature. However, the underlying molecular mechanisms remain poorly understood, and it is unclear how the resistance is maintained without coevolution between hosts and non-adapted pathogens. In this study, we used Phytophthora sojae (Ps), a non-adapted pathogen of Nicotiana benthamiana (Nb), as a model and identified an RXLR

It has been hypothesized that DNA damage has the potential to induce DNA hypermethylation, contributing to carcinogenesis in mammals. However, there is no sufficient evidence to support that DNA damage can cause genome-wide DNA hypermethylation. In this study, we demonstrated that DNA single-strand breaks with 3′ blocked ends (DNA 3′ blocks) not only can reinforce DNA methylation at normally methylated

Hybrid potato breeding based on diploid inbred lines is transforming the way of genetic improvement of this staple food crop, which requires a deep understanding of potato domestication and differentiation. In the present study, we resequenced 314 diploid wild and landrace accessions to generate a variome map of 47,203,407 variants. Using the variome map, we discovered the reshaping of tuber transcriptome

Cotton is the world’s most important natural fiber crop and serves as an ideal model for studying plant genome evolution, cell differentiation, elongation, and cell wall biosynthesis. The first draft genome assembly for Gossypium raimondii, completed in 2012, marked the beginning of global efforts in studying cotton genomics. Over the past decade, the cotton research community has continued to assemble

The intricate interplay between cellular circadian rhythms, primarily manifested in the chloroplast redox oscillations—characterized by diel hyperoxidation/reduction cycles of 2-Cys peroxiredoxins—and the nuclear transcription/translation feedback loop (TTFL) machinery within plant cells, demonstrates a remarkable temporal coherence. However, the molecular mechanisms underlying the integration of these

Although numerous studies have focused on phytohormones in specific organs or tissues at different development stages or under various abiotic and biotic stress conditions, our understanding of the distribution and relative abundance of phytohormones throughout the entire life cycle of plants remains insufficient. Here, we present a phytohormone atlas resource obtained from the quantitative analysis

Maize, a cornerstone of global food security, has undergone remarkable transformations through breeding, yet further increase in global maize production faces mounting challenges in a changing world. In this Perspective paper, we overview the historical successes of maize breeding that laid the foundation for present opportunities. We examine both the specific and shared breeding goals related to diverse

Hydrogen sulfide (H2S) is recognized as an important gaseous signaling molecule, similar to nitric oxide and carbon monoxide. However, less is known about the biosynthetic mechanism of H2S in plants and its role in plant-pathogen interactions. Here, we show that H2S induces the bursts of reactive oxygen species and upregulates the expression of defense-related genes in rice. However, excessive H2S

Plants are able to sense and remember heat stress. An initial priming heat stress enables plants to acclimate so that they are able to survive a subsequent higher temperature. The heat shock transcription factors (HSFs) play a crucial role in this process, but the mechanisms by which plants sense heat stress are not well understood. By comprehensively analyzing the binding targets of all the HSFs,

Wheat (Triticum aestivum) production is vital for global food security, providing energy and protein to millions of people worldwide. Recent advancements in wheat research have led to significant increases in production, fueled by technological and scientific innovation. Here, we summarize the major advancements in wheat research, particularly the integration of biotechnologies and a deeper understanding

Soybean, the fourth most important crop in the world, uniquely serves as a source of both plant oil and plant protein for the world’s food and animal feed. Although soybean production has increased approximately 13-fold over the past 60 years, the continually growing global population necessitates further increases in soybean production. In the past, especially in the last decade, significant progress

As drastic climatic changes significantly impact global agriculture, the importance of conserving and utilizing wild germplasm has gained prominance. In this context, the conservation and sustainable utilization of wild rice germplasm resources have become a high priority. Although efforts to conserve and sustainably utilize wild rice germplasm are underway globally, they are fragmented and require

Herbivore insects deploy salivary effectors to manipulate the defense of their host plants. However, it remains unclear whether small RNAs from insects can function as effectors in regulating plant–insect interactions. Here, we report that a microRNA (miR29-b) found in the saliva of the phloem-feeding whitefly (Bemisia tabaci) can transfer into the host plant phloem during feeding and fine-tune the

Plants defend themselves against pathogens by activating two interconnected layers of immunity: pattern- and effector-triggered immunity. Recent studies shed light on a number of unanswered questions in these pathways and demonstrate a conserved structural basis, in monocots and dicots, of signaling through the EDS1:PAD4:ADR1 module, downstream of Toll/interleukin-1 receptor (TIR) domain-containing

Medicago, a genus in the Leguminosae or Fabaceae family, includes the most globally significant forage crops, notably alfalfa (Medicago sativa). Its close diploid relative Medicago truncatula serves as an exemplary model plant for investigating legume growth and development, as well as symbiosis with rhizobia. Over the past decade, advances in Medicago genomics have significantly deepened our understanding